• WP1: In silico identification of M. truncatula nodule-specific expressed and nodule-induced genes with special focus on the corresponding sugar transporters and glycolysis-related genes.
  • WP2: Determination of the temporal and spatial expression of these genes during the different stages of M. truncatula nodule development.
  • WP3: Examination of the subcellular localization of the corresponding encoded proteins in the nodule, byexpressing protein hybrids with a reporter protein or tagged with an epitope. 
  • WP4: Determination of the substrate specificity and kinetic properties of the targeted sugar transporters.
  • WP5: Analysis of the physiological role of the identified sugar transporters and glycolysis-related genes in the process of SNF, using reverse genetic approaches.
  • WP6: Characterization of the proximal promoters of these genes by in silico analysis as well as by testing the function of the promoter (using a reporter protein) in plants, in order to determine known and new hypoxia-responsive DNA elements.
  • WP7: Identification of nodule-expressed  transcription  factors  capable to recognize hypoxia-responsive DNA elements.
  • WP8: Transfer of knowledge, Dissemination and Outreach activities.
  • WP9: Project Management and Evaluation.